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1º Oficina de Submissão de Artigos da Pará Research Medical Journal

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http://www.santacasa.pa.gov.br/ensino/noticias/detalhe/?id=539

Pará Research Medical Journal
https://prmjournal.org/article/doi/10.4322/prmj.2017.007
Pará Research Medical Journal
Artigo de Pesquisa Pharmaceutical Biotechnology

Study of anti-inflammatory and analgesic properties of 3-benzoyl-propionic acid

Paulo Eduardo Santos Avila, Chubert Bernardo Castro de Sena, Barbarella de Matos Machi, Laine Celestino Pinto, Raquel Carvalho Montenegro, Rosivaldo dos Santos Borges, Gilmara de Nazareth Tavares Bastos, José Luiz Martins do Nascimento

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Abstract

Inflammation is an attempt by the body to remove noxious stimuli and initiate thus a cascade of responses in order to promote healing. There are a variety of inflammatory mechanisms involved in infections, chronic diseases and other tissue damage. Understanding these mechanisms and the search for new anti-inflammatory drugs with greater specificity and fewer side effects, underlying the development and improvement of new protocols and standardization of experimental inflammatory models to understand better these issues. The aim of this study was to evaluate the anti-inflammatory and analgesic activity of 3-benzoyl-propionic acid (3BPA) and its potential toxicological effect. To test the 3BPA as new anti-inflammatory and analgesic drug, the use carrageenan air pouch model 1% by in vitro model of cell culture to test genocytotoxicity. In the in vitro model the 3BPA presented low level of genotoxic and low cytotoxicity risk, shown by comet assay and no damage to the plasma membrane by hemolytic test erythrocytes. In the study of anti-inflammatory activity in vivo by the air pouch method were conducted nitrite dose trials, PGE2 levels and cell migration. To verify analgesic effects of 3BPA drug in vivo tests of abdominal contortions induced by acetic acid and formalin were performed. Regard to the anti-inflammatory activity, 3BPA showed intense activity shown in marked reduction of cell migration and levels of NO, with large populations of neutrophils and reduction of PGE2 values at a dose of 0.5mg/kg. In studies of antinociceptive activity, 3BPA reduced the number of writhes and the time lick the neurogenic and inflammatory phases of the formalin test. The results of this study also advanced substantially with respect to anti-inflammatory and analgesic properties of 3BPA by providing evidence of their likely mechanism of action, through the evaluation of antinociceptive activity, as well as the anti-inflammatory activity in vitro and in vivo, where the 3BPA showed no genotoxic effect.

Keywords

inflammation; analgesia; nonsteroidal anti-inflammatories (NSAIDs); 3-benzoyl-propionic acid.

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