02OUT

1º Oficina de Submissão de Artigos da Pará Research Medical Journal

Na oficina, os candidatos a submissão de artigos receberam informações de como evitar erros que tem sido comuns no momento da submissão desde falhas na redação do artigo, omissão de documentos obrigatórios, até a aprovação no comitê de ética, já que as pesquisas envolvem seres humanos”, esclareceu o Editor Chefe

http://www.santacasa.pa.gov.br/ensino/noticias/detalhe/?id=539

Pará Research Medical Journal
https://prmjournal.org/article/doi/10.4322/prmj.2017.003
Pará Research Medical Journal
Artigo de Pesquisa Farmácia

Avaliação toxicológica do óleo essencial de Cymbopogon citratus (DC) stapf (Capim-Marinho) em camundongos e ratos

Toxicological evaluation of essential oil of Cymbopogon citratus (DC) stapf in mice and rats

Anderson Bentes de Lima, Pedro Iuri Castro da Silva, José da Costa Bastos Junior, Alessandra Souto Cardoso, Luciana Mota Silva, Renata Cunha Silva, Carlos Augusto Lima Barros, Jofre Jacob da Silva Freitas

Downloads: 2
Views: 1179

Resumo

Este trabalho teve como objetivo a avaliação da toxicidade aguda e subcrônica do óleo essencial de Cymbopogon citratus (DC) stapf através da determinação da DL50 em camundongos e a análise dos parâmetros bioquímicos e hematológicos em ratos. A planta é utilizada na medicina popular da região amazônica em diversas doenças. O óleo essencial desta planta apresenta como constituinte principal o citral, (com propriedades ansiolítica, hipnótica, anticonvulsivante e fungicida). A DL50 do óleo essencial foi de 3000±91,32 mg/kg, sendo que este não alterou de maneira significativa os parâmetros bioquímicos e hematológicos em relação ao grupo controle. O valor da DL50 e os resultados observados nos parâmetros hematológicos e bioquímicos sugerem que o óleo essencial de Cymbopogon citratus apresenta uma toxicidade baixa.

Palavras-chave

Cymbopogon citratus; Graminaceae; óleo essencial; toxicidade aguda

Abstract

The aim of this work was the acute and sub-acute toxicological evaluation of the essential oil of Cymbopogon citratus (DC) stapf with the determination of the LD50 in mice and the analysis of their biochemical and haematological parameters in rats. The plant is used in the Amazon folk medicine for several diseases and in their essential oil the citral is the main constituent, presenting anxiolytic, sedative, anticonvulsive and fungicide properties. The LD50 was 3000±91.32 mg/kg, the essential oil do not change significantly the biochemical and hematological parameters when compared with the control group. The LD50 and the biochemical and haematological results have suggested that the essential oil of Cymbopogon citratus presented a low toxicity.

Keywords

Cymbopogon citratus; Graminaceae; essential oil; acute toxicity.

Referências

1. Borrás MRL. 2003. Plantas da Amazônia: medicinais ou mágicas? Plantas comercializadas no Mercado Municipal Adolpho (em busca da identidade regional). Lisboa. Manaus: Ed. Valer. p. 88-89.

2. Maia JGS, Zoghbi MGB, Andrade EHA, Santos AS. Lista de Plantas Aromáticas. In: Maia JGS, Zoghbi MGB, Andrade EHA. Aromáticas na Amazônia e seus óleos essenciais. Belém: MPEG; 2001. p. 76-77. Coleção Adolpho Ducke.

3. Leite CJ, Sousa JP, Medeiros JA, Conceição ML, Santos Falcão Silva V, Souza EL. Inactivation of Escherichia coli, Listeria monocytogenes, and Salmonella Enteritidis by Cymbopogon citratus D.C. Stapf. Essential Oil in Pineapple Juice. J Food Prot. 2016 fev;79(2):213-9. PMid:26818981. http://dx.doi.org/10.4315/0362-028X.JFP-15-245.

4. Van Den Berg ME. 1993. Plantas medicinais na Amazônia: Contribuição ao seu conhecimento sistemático. 2. ed. Belém: Museu Paraense Emílio Goeldi.

5. Devi RC, Sim SM, Ismail R. Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum. J Smooth Muscle Res. 2011;47(5):143-56. PMid:22104376. http://dx.doi.org/10.1540/jsmr.47.143.

6. Coimbra R. Manual de fitoterapia. 2. ed. Belém: CEJUP; 1994.

7. Fernandes C, Souza H, Oliveria G, Costa J, Kerntopf M, Campos A. Investigation of the mechanisms underlying the gastroprotective effect of Cymbopogon citratus essential oil. J Young Pharm. 2012;4(1):28-32. PMid:22523457. http://dx.doi.org/10.4103/0975-1483.93578.

8. Sagradas J, Costa G, Figueirinha A, Castel-Branco MM, Silvério Cabrita AM, Figueiredo IV, et al. Gastroprotective effect of Cymbopogon citratus infusion on acute ethanol-induced gastric lesions in rats. J Ethnopharmacol. 2015;15:134-8. PMid:26160747. http://dx.doi.org/10.1016/j.jep.2015.07.001.

9. Adeneye AA, Agbaje EO. Hypoglycemic and hypolipidemic effects of fresh leaf aqueous extract of Cymbopogon citratus Stapf. in rats. J. Ethnopharmacol. 2007;25;112(3):440-4.

10. Paranagama PA, Abeysekera KHT, Abeywickrama K, Nugaliyadde L. Fungicidal and anti-aflatoxigenic effects of the essential oil of Cymbopogon citratus (DC.) Stapf. (lemongrass) against Aspergillus flavus Link. Isolated from store rice. Lettes Appl Microbiol. 2003;37:86-90.

11. Boukhatem MN, Ferhat MA, Kameli A, Saidi F, Kebir HT. Lemon grass (Cymbopogon citratus) essential oil as a potent anti-inflammatory and antifungal drugs. Libyan J Med. 2014; 19;9:25431.

12. Maia JGS, Zoghbi MGB, Andrade EHA, Santos AS, Silva MHL, Luz AIR, et al. Constituents of the essential oil of Piper aduncum L. growing wild in the Amazon region. Flavour Fragrance J. 1998;13(4):269-72. http://dx.doi.org/10.1002/(SICI)1099-1026(1998070)13:4<269::AID-FFJ744>3.0.CO;2-A.

13. Adams RP. Identication of Essential Oil Components by Gas majoritário do oleo Chromatography/Quadrupole Mass Spectroscopy. Carol Stream, IL: Allured Publishing; 2001.

14. Malone MH, Robichaud RC. A hippocratic screen for pure or crude drug materials. Lloydia. 1962;25:320-32.

15. Miller LC, Tainter ML. Estimation of the LD50 and its error by means of logarithmic probit graph paper. Proc Soc Exp Biol Med. 1944;57(2):261-4. http://dx.doi.org/10.3181/00379727-57-14776.

16. Jong WH, Kruese ED, Vos JG, Van Loveren H. Detection of immunotoxicity of benzo[a]pyrene in a subscute toxicity study after oral exposure in rats. Toxicol Sci. 1999;50(2):214-20. PMid:10478857. http://dx.doi.org/10.1093/toxsci/50.2.214.

17. Toyoda K, Shibutani M, Tamura T, Koujitani T, Uneyama C, Hirose M. Repeated dose (28 days) oral toxity study of flutamide in rats based on the draft protocol for the “Enhanced OECD Test Guideline 407” for screening for endocrine disrupting chemicals. Arch Toxicol. 2000;74(3):32-127. PMid:10876997. http://dx.doi.org/10.1007/s002040050664.

18. Vijayalakshmi T, Muthulakshmi V, Sachdanandam P. Toxic studies on biochemical para meters carried out in rats with serankottai nei, a siddha drug-milk extract of Semecarpus anacardium nut. J Ethnopharmacol. 2000;69(1):9-15. PMid:10661878. http://dx.doi.org/10.1016/S0378-8741(99)00020-3.

19. Yanagisawa T, Maemura S, Sasaki H, Endo T, Okada M, East PW, et al. Subacute and chronic toxicity studies of tryethylenetetramine dihydrochloride (TJA-250) by oral administration to F-344 rats. J Toxicol Sci. 1998;23(Suppl 4):619-42. PMid:9836186. http://dx.doi.org/10.2131/jts.23.SupplementIV_619.

20. Mota AS, Lima AB, Albuquerque TL, Silveira TS, Nascimento JL, Silva JKR, et al. Antinociceptive Activity and Toxicity Evaluation of the Fatty Oil from Plukenetia polyadenia Mull. Arg. (Euphorbiaceae). Molecules. 2015 abr 30;20(5):7925-39. PMid:25942373. http://dx.doi.org/10.3390/molecules20057925.

21. Costa CA, Bidinotto LT, Takahira RK, Salvadori DM, Barbisan LF, Costa M. Cholesterol reduction and lack of genotoxic or toxic effects in mice after repeated 21day oral intake of lemongrass (Cymbopogon citratus) essential oil. Food Chem Toxicol. 2011;49(9):2268-72. PMid:21693164. http://dx.doi.org/10.1016/j.fct.2011.06.025.

22. Organization of Economic Co-operation and Development. The revised up-and-down procedure: a test method for determining the acute oral toxicity of chemicals. Rockville: NIH Publication; 2001. no 02-4501.

23. Viana GS, Vale TG, Pinho RS, Matos FJ. Antinociceptive effect of the essential oil from Cymbopogon citratus in mice. J Ethnopharmacol. 2000;70(3):323-7. PMid:10837994. http://dx.doi.org/10.1016/S0378-8741(99)00168-3.

24. Carlini EA, Contar DP, Silva-Filho AR, Silveira Fo NG, Frochtengarden ML, Bueno OF. Pharmacology of lemongrass (Cymbopogon citratus Stapf). I. Effects of teas prepared from the leaves on laboratory animals. J Ethnopharmacol. 1986;17(1):37-64.

25. Leite JR, Seabra MP, Maluf E, Assolant K, Suchecki D, Tufik S, et al. Pharmacology of lemongrass (Cymbopogon citratus Stapf). III. Assessment of eventual toxic, hypnotic and anxiolytic effects on humans. J Ethnopharmacol. 1986;17(1):75-83. PMid:2429120. http://dx.doi.org/10.1016/0378-8741(86)90074-7.

26. Ress NB, Hailey JR, Maronpot RR, Bucher JR, Travlos GS, Haseman JK, et al. Toxicology and carcinogenesis studies of microencapsulated citral in rats and mice. Toxicol Sci. 2003 fev;71(2):198-206. PMid:12563105. http://dx.doi.org/10.1093/toxsci/71.2.198.

59d26b2f0e8825f910fbe3d7 prmjournal Articles
Links & Downloads

PRMJ

Share this page
Page Sections